Table 3.
Isolation of T. gondii by bioassay in mice
| Sample ID | Host | Location | Date sample collected | MAT titer | Days between collection and inoculation | Positive in cell culture/mice useda | Isolate ID |
|---|---|---|---|---|---|---|---|
| 17 | wtd | TN | 10/8/2011 | ≥ 8192 | 6 | 3/3 | TgMnkTn17 |
| 40 | wtd | SC | 10/15/2011 | ≥ 8192 | 16 | 2/3 | TgWtdSc40 |
| 43 | wtd | SC | 10/15/2011 | ≥ 8192 | 16 | 2/3 | TgWtdSc43 |
| 60 | wtd | SC | 10/15/2011 | ≥ 8192 | 16 | 3/3 | TgWtdSc60 |
| 78 | wtd | SC | 11/13/2011 | ≥ 8192 | 9 | 2/2 | TgWtdSc78 |
| 88 | wtd | SC | 11/13/2011 | ≥ 8192 | 9 | 2/2 | TgWtdSc88 |
| 98 | wtd | SC | 11/13/2011 | 2048 | 9 | 2/2 | TgWtdSc98 |
| 99 | wtd | SC | 11/13/2011 | 2048 | 9 | 2/2 | TgWtdSc99 |
| 110 | wtd | SC | 11/13/2011 | ≥ 8192 | 9 | 2/2 | TgWtdSc110 |
| 113 | wtd | SC | 11/13/2011 | ≥ 8192 | 9 | 2/2 | TgWtdSc113 |
| 122 | coyote | TN | 1/21/2012 | 512 | 12 | 1/1 | TgCyTn122 |
| 142 | coyote | TN | 2/2/2012 | 4096 | 14 | 2/2 | TgCyTn142 |
| 194 | feral hog. | NC | 1/28/2013 | 4096 | 16 | 2/3 | TgHogNc194 |
| 227 | feral hog | NC | 4/9/2013 | 128 | 16 | 2/3 | TgHogNc227 |
| 335 | wtd | TN | 11/12/2013 | 128 | 9 | 1/2 | TgWtdTn335 |
| 372 | wtd | TN | 12/9/2013 | ≥ 8192 | 4 | 2/2 | TgWtdTn372 |
| 387 | wtd | TN | 12/9/2013 | ≥ 8192 | 4 | 2/2 | TgWtdTn387 |
| 399 | wtd | TN | 12/15/2013 | 2048 | 8 | 1/2 | TgWtdTn399 |
| 452 | feral hog | NC | 1/16/2014 | 128 | 18 | 2/3 | TgHogNc452 |
Abbreviations: NC North Carolina, SC South Carolina, TN Tennessee, wtd white-tailed deer
aNumber of mice from which T. gondii was successfully isolated and expanded in cell culture versus total number of mice used for bioassay