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. 2017 Oct 23;10:506. doi: 10.1186/s13071-017-2401-4

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Effect of L. major and L. tropica SLA on NO production by macrophages. The J774 macrophages cells were cultured in presence of L. tropica SLA (a), L. major SLA (b), and stimulated according to the same protocol than promastigotes. The supernatants were collected and nitrite concentrations were evaluated by Griess reaction after 24 h post-infection. Results are expressed as mean ± SEM from three independent experiments, each performed in duplicate. Data were analyzed using one way ANOVA test with Tukey’s post-hoc test for multiple comparisons. ***P < 0.001