Fig. 5.

Leishmania major and L. tropica promastigotes and amastigotes sensitivity to exogenous NO. Viability of L. tropica promastigotes (a) and viability of L. major promastigotes (b) and amastigotes (c, d) was evaluated after 24 h incubation with different concentrations of SNAP and its control (NAP). In all experiments, the addition of NAP at the same concentrations as SNAP did not impact the viability of parasites (100% viability). Results are expressed as mean ± SEM from six independent experiments, each performed in duplicate. The NO release by SNAP was measured in the culture supernatant for each concentration. NO concentration ranges: a and b: 17.26–55.20 μM, c and d: 14.75–44.67 μM. Data were analyzed using one way ANOVA test with Tukey’s post-hoc test for multiple comparisons.**P < 0.01, ***P < 0.001. Abbreviation: ns: not significant