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Morphology of colony biofilms cultivated on filters. a 3-day-old BR-F colonies cultivated on GMA medium with 0.3% agar in the absence or presence of a filter. b Aerial view of root parts of colony biofilms of the BR-F-Arg1p-GFP strain cultivated as in (a). BF, bright field; GFP, GFP fluorescence. c Side view of a vertical cross-section of root parts of colony biofilms of the BR-F-Arg1p-GFP strain cultivated as in (a) using 2PE-CM. Green, GFP fluorescence, red, filter autofluorescence
