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ROS level of CFs in different experimental groups. (a) Intracellular ROS accumulation in different groups stained by DHE. Representative pictures were shown. (b) Mean fluorescence intensity was detected by ImageJ software. Data were presented as the mean ± SEM (N = 3). ∗∗P < 0.01 versus NG, ##P < 0.01 versus HG, ^^P < 0.01 versus HG + Alda-1.
