Figure 1.

The effects of ghrelin on the cell viability of H₂O₂-treated HLECs. (a) HLECs were incubated with different concentrations of ghrelin (10⁻⁹–10⁻⁶ M) for 24 h. (b) HLECs were incubated with different concentrations of H₂O₂ (50–800 μM) for 24 h. (c) HLECs were preincubated with ghrelin (10⁻⁹–10⁻⁶ M) for 12 h before being treated with 100 μM H₂O₂ for 24 h. Cell viability was assessed via MTT assay. (d) HLECs were detected by microscopy. The results were represented as the mean ± SEM (n = 3) from three independent experiments. ^αP < 0.01, compared with the untreated control group; ^∗P < 0.05, ^∗∗P < 0.01, compared with the H₂O₂-treated group.