Figure 2.

Ghrelin prevented H₂O₂-induced cellular apoptosis in HLECs. HLECs were preincubated with ghrelin (10⁻⁹–10⁻⁶ M) for 12 h before being treated with 100 μM H₂O₂ for 24 h. (a) Apoptosis of HLECs as detected by flow cytometry. The results were represented as the mean ± SEM (n = 3) from three independent experiments. (b) Ghrelin significantly decreased the apoptosis rate of HLECs. ^αP < 0.01, compared with the untreated control group; ^∗P < 0.05, ^∗∗P < 0.01, compared with the H₂O₂-treated group.