Fig. 4. In vitro activity-based protein profiling of GH29 α-l-fucosidases. (A) Labeling with ABP 1 of recombinant α-l-fucosidase from Bacteroides thetaiotaomicron 2970. (B) In vitro labeling of lysate of spleen from a Gaucher disease patient, α-(1-2,3,4)-fucosidase from Xanthomonas sp. and α-(1-6)-fucosidase from Elizabethkingia miricola with ABP 2. (C) In vitro labeling of human healthy and Gaucher disease spleen. (D) Direct labeling of GH29 α-l-fucosidases with green-fluorescent ABP 1 and retaining β-glucosidases GBA, GBA2 and GBA3 with red-fluorescent JJB75.18 The location of albumin autofluorescence is designated on each gel.
