Fig. 1.

Schematic diagram of mutations of Gm-JAG1 (Glyma20g25000) in the high-density mutant library of ‘Enrei’. Glyma20g25000 consists of 2,436 bp (black line), including 771 bp coding sequences connected by 5 exons. The first exon consists of a sequence of 2 bases, and the remaining exons are included in 4 boxes. The mutant line ‘EnT-0685’ and a natural variation of ln (ninth aspartic acid changed to histidine: D9H) have a respective unique mutation on the ERF-associated amphiphilic repression (EAR) motif (8aa-LDLNNLP-14aa; black box). Only the progenies of ‘EnT-0541’ and ‘EnT-0685’ with the mutant genotype showed narrow leaflet shape like the ln genotype. The detailed information of 1 null and 8 missense mutant lines and screening method has been described in Tsuda et al. (2015).