Figure 3. Failed PCV-13 vaccine efficacy in the context of VAD.
(A) Bacterial titers and (B) survival are shown for PCV-13 vaccinated (N = 29–30 per group) and unvaccinated (N = 12–15 per group) VAD and control animals following challenge with pathogenic pneumococcus. All mice were infected with 106 CFU of serotype T4. Results are shown for two experiments combined. CON=control animals. Statistical significance was determined using a Mann-Whitney U test for comparisons of bacterial titers. Significant differences were observed between vaccinated and unvaccinated mice and between VAD and control mice. Significant differences in survival were evaluated using a Mantel-Cox test.