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. Author manuscript; available in PMC: 2017 Oct 24.
Published in final edited form as: Arch Toxicol. 2016 Nov 8;91(5):2245–2261. doi: 10.1007/s00204-016-1885-6

Figure 1. APAP overdosing induces hepatic RNA and protein expression of Panx1.

Figure 1

Mice (n = 5 per group and per time point) were injected 300 mg/kg APAP or saline (SAL) followed by sampling at different time points (0, 1, 6 and 24 hours). (a) RNA was extracted and subjected to RT-qPCR analysis of Panx1 using the primers as depicted in Table 1. Relative alterations in RNA levels were calculated according to the 2-ΔΔCq formula, where the average expression of untreated animals at the 0 hour time point is set to 1. (b) Hepatic protein levels of Panx1 were assessed by immunoblot analysis, normalized against the total protein content and expressed as relative alteration compared to untreated animals. Data were expressed as means ± SEM, with **p < 0.01, ***p < 0.001 and ****p < 0.0001 compared to vehicle control at indicated time points.