. Author manuscript; available in PMC: 2017 Oct 24.

Published in final edited form as: Arch Toxicol. 2016 Nov 8;91(5):2245–2261. doi: 10.1007/s00204-016-1885-6

Table 1. Primers used for RT-qPCR analysis.

Gene	Assay ID	Accession number	Assay location	Amplicon size (base pairs)	Exon boundary
Panx1	Mm00450900_m1	NM_019482.2	986	70	3-4
18S	Hs99999901_s1	X03205.1	604	187	1-1
Actb	Mm00607939_s1	NM_007393.3	1233	115	6-6
B2m	Mm00437762_m1	NM_009735.3	111	77	1-2
Gapdh	Mm99999915_g1	NM_008084.2	265	107	2-3
Hmbs	Mm01143545_m1	NM_013551.2	473	81	6-7
Ubc	Mm02525934_g1	NM_019639.4	370	176	2-2

Assay identification (ID) and accession number of target genes are indicated (18S, 18S ribosomal RNA; Actb, β-actin; B2m, β-2-microglobulin; Gapdh, glyceraldehyde 3-phosphate dehydrogenase; Hmbs, hydroxymethylbilane synthase; Panx1, pannexin1; Ubc, ubiquitin C).