Table 2. Panx1 glycosylation status after APAP overdosing.

Time point	Treatment	Total Panx1 normalized to 0 hour	Gly0 (%)	Gly1 (%)	Gly2 (%)
0 hour	/	1.00 ± 0.14	49.5 ± 1.4	13.9 ± 1.9	36.6 ± 3.3
1 hour	SAL	1.21 ± 0.10	58.6 ± 2.0	14.0 ± 1.9	27.3 ± 3.0
	APAP	0.85 ± 0.10	48.8 ± 3.5	5.2 ± 1.7***	46.0 ± 4.2**
6 hours	SAL	1.18 ± 0.11	54.1 ± 1.6	10.4 ± 1.3	35.6 ± 2.4
	APAP	2.08 ± 0.27	11.4 ± 5.7****	4.0 ± 1.0*	84.5 ± 6.6****
24 hours	SAL	1.96 ± 0.19	63.0 ± 1.8	18.1 ± 1.7	18.8 ± 0.7
	APAP	3.31 ± 0.20	5.7 ± 1.8****	3.8 ± 0.3****	90.5 ± 1.9****

Mice (n = 5) were injected 300 mg/kg APAP or saline (SAL) followed by sampling at different time points (0, 1, 6 and 24 hours). Hepatic protein levels of Panx1 were assessed by immunoblot analysis where Panx1 was detected as 3 signals, representing the non-glycosylated core (Gly0), the high-mannose (Gly1) and the complex glycosylated species (Gly2). Data were expressed as means ± SEM, with *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 compared to vehicle control at indicated time points.