Fig. 3.

Screening of complementary DNA oligonucleotides for efficiency of affinity capture of GFP mRNA. (a) Schematic of the 5′ region of the GFP open reading frame (ORF; from the start codon through codon 69). Approximate site of complementarity for each tested DNA oligonucleotide is indicated. (b) Northern blot analysis of GFP mRNA (target RNA) captured from total purified yeast RNA using different complementary DNA oligonucleotides (oligos) either alone or in combination. Oligonucleotide numbers correspond to the labels in (a). Percent target RNA remaining in the supernatant or captured in the eluate are quantified relative to the input and are indicated below