Reagent	Volume	Final concentration
Yeast lysate + TE buffer	3 mL	N/A
5 M LiCl	500 μL	500 mM LiCl
10% SDS	250 μL	0.5% SDS
0.5 M EDTA pH 8.0	500 μL	50 mM EDTA pH 8.0
1 M Tris–HCl pH 7.5	50 μL	10 mM Tris–HCl pH 7.5
100% formamide	700 μL	14% formamide
Fungal protease inhibitor	5 μL	N/A