(A) A LAMP assay was performed on 2 μl of 10-fold dilutions of Vero cell supernatants containing 6.4 × 105 PFU/ml. Virus input: 1280 PFU (black circles), 128 PFU (black triangles), 12.8 PFU (black squares), 1.28 PFU (black diamonds), 0.128 PFU (black crosses), and 0.0128 PFU (no symbols; line below threshold). (B) A LAMP assay was performed on 10-fold dilutions of Zika virus genome copies: 105 copies (black circles), 104 copies (black triangles), 103 copies (black squares), 102 copies (black diamonds), 10 copies (black crosses), and 1 copy (line, no symbols). (A and B) Control infected Vero cell RNA (positive control; red circles) and no RNA (no symbols; line below threshold). Results are representative of a minimum of six replicates of each dilution series. X axis, minutes to LAMP amplicon incorporation of fluorescent dye; y axis, RFUs.