Table 1.
Gene name | Forward sequence (5′-3′) | Reverse sequence (5′-3′) | Probe sequence (5′-3′) |
---|---|---|---|
Smart GAPdH | CACATGGCCTCCAAGGAGTAA | TGAGGGTCTCTCTCTTCCTCTTGT | VIC/CTGGACCACCAGCCCCAGCAAG |
VZV ORF 68 | GTACATTTGGAACATGCGCG | TCCACATATGAAACTCAGCCC | FAM/AAAACAAGAAACCCTACGCCCGC |
Unique HSV-1a | TGGTATTGCCCAACACTTTCC | GCGCCAGGCACACACAT | FAM/CGTGTCGCGTGTGGT/BHQ |
Amplifies the non-coding region of the HSV-1 genome between UL42 and UL43