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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Cancer Res. 2017 Aug 15;77(19):5228–5235. doi: 10.1158/0008-5472.CAN-17-0320

Figure 4.

Figure 4

CPSF1 regulates AR-V expression. A. Western blot with lysates from 22Rv1-undup3 transfected with four independent and pooled siRNAs. Blots were probed with antibodies to CPSF1 or ERK-2 (loading control). B. Western blot with lysates from 22Rv1-undup3 cells transfected with CPSFS1 siRNA as in panel A, probed with antibodies to AR, ERK-2 (loading control), or AR-V7. C. Growth of 22Rv1-undup3 cells transfected as with CPSF1 siRNA was assessed under androgen replete (charcoal stripped serum+1 nM DHT) or androgen deplete (charcoal-stripped serum+ethanol, vehicle control) conditions by crystal violet staining. Data points represent means (n = 6, error bars = 95% confidence intervals). **** p ≤ 0.0001,* p≤ 0.05, ns p>0.05, unpaired t-test. D. Model for AR splicing decisions regulated by CPSF1-dependent selection of a single PAS in AR intron 3.