At 4.5 hours after resuscitation, Evans blue dye (EBD)-albumin extravasation was significantly higher in hemorrhaged mice adjuvantly treated with vehicle (Veh) than in sham mice, and significantly lower in mice adjuvantly treated with C23, as indicated by (A) visual detection of EBD-albumin on the surface of the lungs (representative images) and (B) spectrophotometric quantification of EBD after extraction with formamide. (C) Compared with sham mice, ICAM-1 protein expression was significantly elevated in the lungs of mice adjuvantly treated with vehicle and significantly decreased in mice adjuvantly treated with C23. Representative Western blot showing three samples per group. Data are presented as means ± SE (n=6 per group). *p < 0.05 vs. sham, #p < 0.05 vs. vehicle; one-way ANOVA followed by Student-Newman-Keuls test.