Figure 4. IL-2- and αKG-sensitive events impact CTCF.

(A) A heatmap representing genome-wide CTCF enriched ChIP-seq peaks from biological replicates of primary CD4⁺ T cells polarized in Th1 conditions that were exposed to high environmental IL-2, low IL-2, or low IL-2 with αKG conditions for two days. (B) Histograms representing differential CTCF ChIP-seq peak read depth enrichment between high IL-2 compared to low IL-2 conditions (left) or low IL-2 with αKG relative to low IL-2 conditions (right). The distribution of the log ratio of peak read enrichment is displayed. (C) CTCF-ChIP-seq tracks from two biological replicates of CD4⁺ Th1 cells exposed to high IL-2, low IL-2, or low IL-2 with αKG displayed with the UCSC genome browser. Differential CTCF peaks are highlighted with a blue arrow. (D, E) qRT-PCR analysis of transcripts from primary CD4⁺ T cells polarized in Th1 conditions and transfected with a control siRNA to GFP (black bar), (D) an siRNA to Ctcf (grey bar) or (E) an siRNA to Rad21 (dark grey bar). The control GFP siRNA is the same for Figure S3E and 4D. The n= at least 3 independent biological replicates for each gene. Error bars represent SEM and an unpaired student t-test was performed with p values indicated (* ≤0.05, **≤0.001, and ***≤0.0001). See also Fig. S4–7.