Table 3.

Rates obtained from modeling the FRET data using KinTek Global Explorer

Step	Reactiona	Constantb	Non-extendable DNA		Extendable DNA
			WT	E288K	WT	E288K
DNA Binding	E + D → ED	KD (DNA)#	5	19	5	19
	ED → E + D
dNTP Binding	ED → N + EDN	Kd(dNTP)*	3.6±0.5	5.8±1.2	3.6±0.5	5.8±1.2
	EDN → ED + N
Fingers Closing	EDN → END	k+3	129±18	114±8	84±44	90±21
	END → EDN	k−3	84±9	9±2	148±27	9±3
Non-covalent Step	END → NDE	k+4	18±1	6.5±0.3	17±13	10±2
	NDE → END	k−4	0.59±0.05	0.26±0.03	1.4±2.0	41±13
Polymer-ization	NDE → EP	k+5			10.9±0.3	22.6±1.1
	EP → NDE	k−5			0	0
Post Chemistry	EP → E + P	k+6			0.45±0.11	0.3±0.2
	E + P → EP	k−6⁋			0.02±0.07	0.03±0.2

^aReaction as modeled in KinTek Explorer. E represents enzyme, D represents DNA substrate, N represents nucleotide, and P represents product.

^bRate constants all have units of s⁻¹ except^K has units of μM⁻¹s⁻¹. ^# has units of nM and * has units of μM.