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. 2017 Oct 24;7:13902. doi: 10.1038/s41598-017-12584-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Long-lasting immunological memory induced by pre-tumor lysate treatment. Mice were treated with lysate >100 days prior to tumor inoculation with 5 × 10⁵ glioma cells of the corresponding cell line. In both glioma models, GL261 and CT2A, lysate treated mice were compared to PBS treated control animals. Statistical significance was calculated by Log-rank test, **p < 0.01; ****p < 0.0001.