Fig. 6.

F/R rescues subendothelial CC production in vivo. a, c Ldlr ^−/− mice were fed a HFD for 1 week with simultaneous IP injections with F/R or PBS (control) every other day. b, d Ldlr ^−/− mice were kept on HFD for 1 week before initiation of IP injections with F/R or PBS (control) every other day for an additional week on HFD. a, b Aortic root sections were subjected to PL to visualize CC. (scale bar = 500 μm) c, d The aortic arch was used for analysis by SEM (luminal surface) and TEM. Yellow arrows show subendothelial protrusions from the aortic surface. (Error bars represent SEM, *p < 0.05, representative images of n = 8, IP: intraperitoneal injection, C: collagen, CC: cholesterol crystal, SMC: smooth muscle cell, E: elastin layer, EC: endothelial cell)