Table 3.
Primary antibodies employed
| Antigen | Supplier | Previous use in human | Dilution | Species |
|---|---|---|---|---|
| NRXN1α+β | Santa Cruz. Sc14334 | Western blot: Kim et al. (2008). | 1/300 | Goat polyclonal |
| Immunohistochemistry: Zhang et al. (2013). | ||||
| NRXN2α | AbCam. Ab34245 | Immunohistochemistry: Borsics et al. (2010). | 1/2000 | Rabbit polyclonal |
| NRXN3α+β | Sigma-Alldrich Prestige. HPA 002727 | Tested by human protein atlas including immunohistochemistry. | 1/300 | Rabbit polyclonal |
| CASK | AbCam. ab126609 | Immunohistochemistry: Wei et al. (2014). | 1/350 | Rabbit monoclonal |
| KHDBRS1 | Santa Cruz. Sc333 | 1/1500 | Rabbit polyclonal | |
| KHDBRS2 | Dr Peter Scheiffele | 1/10 000 | Rabbit polyclonal | |
| KHDBRS3 | Dr Peter Scheiffele | 1/5000 | Guinea pig polyclonal | |
| PAX6 | Covance, PRB-278P | Immunohistochemistry: Bayatti et al. (2008). | 1/1500 | Rabbit polyclonal |
| TBR1 | AbCam Ab31940 | Immunohistochemistry: Bayatti et al. (2008). | 1/1500 | Rabbit polyclonal |
| GAP43 | Sigma-Alldrich G9264 | Immunohistochemistry: Bayatti et al. (2008). | 1/10 000 | Mouse monoclonal |
| SYP | Sigma-Alldrich | Immunohistochemistry: Bayatti et al. (2008). | 1/1000 | Mouse monoclonal |
For antigenic sites of anti-NRXN antibodies, see Supplementary Fig. 1. anti-NRXN1 has also been tested against recombinant mouse NRXN1 expressed in a human kidney cell line (Cheng et al. 2009).