Fig. 6.

Ectopic expression of TRN2 mimics the crc mutant phenotype. a–d Morphology of crc-1 (a), pCRC::TRN2 (b), pCRC::TRN2 knu-1 (c), and NPA-treated pCRC::TRN2 knu-1 (d) fruits. Top: close-up views of fruit tips. Bottom: shapes of whole fruits. Arrowheads indicate stigma structures. Two stigma-like structures were observed in the crc-1 mutant as well as pCRC::TRN2 due to carpel separation. Images are shown at the same magnification. e Quantification of mutant phenotypes. p-values were calculated by χ ² test (n = 30). f, g Longitudinal sections of mock-treated pCRC::TRN2 knu-1 (f) and NPA-treated pCRC::TRN2 knu-1 (g) fruits. Asterisks indicate carpels. h, i Scanning electron micrograph of pCRC::TRN2 knu-1 fruits with reiterations of floral organs. pCRC::TRN2 knu-1 (h) and NPA-treated pCRC::TRN2 knu-1 (i) fruits. j–m Floral meristem activity marker, WUS mRNA, in longitudinal sections of crc-1 (j), pCRC::TRN2 (k), pCRC::TRN2 knu-1 (l), and NPA-treated pCRC::TRN2 knu-1 (m) floral buds at developmental stage 6. Images are shown at the same magnification. Bars = 1 cm in a–d; 500 µm in f, g; 1 mm in h, i; and 50 µm in j–m