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Characterization of tumor-organoids for neo-angiogenesis-related markers. (A) Immunofluorescence analysis of the resulting tumor-organoids and human HCC tissue, white arrows; strong double positive cells in human HCC tissue, GPC-3; red, nuclei; blue, VEGFR2; green. Scale bar 200 µm. (n = 3). (B) Quantitative PCR analysis of VEGFR2, VEGF, HIF-α in human tumor-organoids and controls at 24 h after self-assembly. Results represent mean ± s.d., (n = 3) * p < 0.05.
