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. 2017 Oct 24;7:13888. doi: 10.1038/s41598-017-14304-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PCR detection of B. microti from CF‒ or DA‒treated mice on day 52 post-infection. (a) in blood DNA samples: G1, untreated; G2, 25 mg/kg DA; G3, 20 mg/kg CF; G4, 15 mg/kg CF and 10 mg/kg DA combination; G5, 10 mg/kg CF and 15 mg/kg DA combination. (b–f) in tissue DNA samples: M, 100 bp size marker; PC, positive control; Br., brain; Sp., spleen; Li., liver; Ki., kidney; He., heart, NC, negative control. The double bands observed with some of the positive controls represent amplicons of the first and second PCR. Only a single band was observed when the concentration of template DNA was reduced (data not shown). (g) Infectivity of B. microti in RBC-transfused mice. Parasitemia was calculated by counting infected RBCs among 500 RBCs.