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Effect of EMD on osteogenic differentiation of PDLSCs. (A) After 21 d of incubation in the induction medium added with 30 and 60 µg/ml of EMD, mineralized deposits was evaluated by Alizarin red S staining. (B) The deposition of Alizarin staining was extracted, and the absorbance value of released dye was determined at 562 nm using a microplate reader. *P< 0.05 and **P < 0.01 vs. the control.
