Figure 4.

Comparison of interfaces in naturally occurring and fixed and flexible backbone designed homo‐oligomers. (A‐C) Evaluation of backbone complementarity, interface area and the ratio of interface area between neighboring subunits and total surface area per subunit between naturally‐occurring oligomers (N) and cyclic conformations sampled utilizing different de novo building blocks. Cyclic homo‐oligomer conformations were generated with either fixed‐backbone (fix‐bb) or flexible‐bb (flex‐bb) design method. Only backbone atoms were included in all calculations. Orange: first quartile to median. Gray: median to third quartile. (D–F) Same evaluation as (A–C) but including all atoms rather than just the backbone. (G,H) Same evaluation as D and F for all of the experimentally characterized designs. (I) Evaluation of interface polarity for all of the experimentally characterized designs