TABLE 1.
Overview of RT-PCR methods used for ZIKV RNA detection in EQA
| Method (readout) | Authors, manufacturer, or source, yr (reference no.) | No. of submissions |
|---|---|---|
| In-house conventional RT-PCR | Faye et al., 2008 (16) | 1 |
| Balm et al., 2012 (17) | 1 | |
| Moureau et al., 2007 (18) | 1 | |
| Patel et al., 2013 (19) | 1 | |
| Own design | 1 | |
| Scaramozzino et al., 2001 (20) | 3 | |
| In-house quantitative real-time RT-PCR (no. of RNA copies/ml) | Corman et al., 2016 (21) | 3 |
| Real-time RT-PCR (CT value) | ||
| In-house | Corman et al., 2016 (21) | 3 |
| Faye et al., 2013 (22) | 13 | |
| Lanciotti et al., 2008 (2) | 17 | |
| Pyke et al., 2014 (23) | 1 | |
| Tappe et al., 2014 (24) | 1 | |
| Own design | 8 | |
| CDC Trioplex (25) | 4 | |
| Commercial | Altona (26) | 16 |
| Clonit (27) | 1 | |
| Fast Track Diagnostics (28) | 1 | |
| Genesig (29) | 1 | |
| Liferiver (30) | 2 | |
| Roche (31) | 2 | |
| Sacace Biotechnologies (32) | 1 | |
| In-house RT–loop-mediated isothermal amplification (time [in minutes]) | Own design | 3 |