FIG 2 .

Junctional proteins JAM-A and ALCAM are increased on HIV⁺ CD14⁺ CD16⁺ monocytes. (A) Representative graph showing gating strategy on HIV⁺ and HIV^exp CD14⁺ CD16⁺ monocytes to examine JAM-A on both subsets. Representative histograms show JAM-A (green) and IgG1 isotype-matched control antibody (black) expression on HIV⁺ and HIV^exp CD14⁺ CD16⁺ monocytes. ΔMFI (change in mean fluorescence intensity) is calculated by subtracting JAM-A MFI from IgG1 isotype MFI. (B) Surface JAM-A on HIV⁺ and HIV^exp CD14⁺ CD16⁺ monocytes (n = 7). (C) Representative graph showing gating strategy on HIV⁺ and HIV^exp CD14⁺ CD16⁺ monocytes to examine ALCAM on both subsets. Representative histograms show ALCAM (pink) and IgG1 isotype-matched control antibody (black) expression on HIV⁺ and HIV^exp CD14⁺ CD16⁺ monocytes. ΔMFI is calculated by subtracting ALCAM MFI from IgG1 isotype MFI. (D) Surface ALCAM on HIV⁺ and HIV^exp CD14⁺ CD16⁺ monocytes (n = 7). Data are represented as mean ± SEM. Significance was determined by Wilcoxon’s signed-rank test. Significance is compared to baseline unless indicated otherwise. *, P < 0.05.