TABLE 1 .

Genes identified by TraDIS that affect capsule production in PA45B

Gene name	Locus tag	Mutated gene	Capsule synthesis disruptedb	Genome positon	Product	Log FCc
mprA	PA45B_3600	+	+	2953533–2954063	DNA-binding transcriptional repressor	7.28
neuAa	PA45B_3235	−	NA	3339754–3340863	N-Acylneuraminate cytidylyltransferase	7.21
kpsCa	PA45B_3240	−	NA	3336039–3337244	Capsule polysaccharide export protein	7.19
kpsEa	PA45B_3243	−	NA	3330382–3331530	Capsule polysaccharide export protein	7.18
kpsDa	PA45B_3242	+	+	3331554–333230	Capsule polysaccharide export protein	7.18
kpsMa	PA45B_3231	−	NA	3344548–3345324	Capsule polysaccharide transport protein	7.16
kpsTa	PA45B_3232	−	NA	3346382–3346918	Capsule polysaccharide transport ATP-binding protein	7.12
kpsSa	PA45B_3239	−	NA	3336039–3337244	Capsule polysaccharide export protein	7.10
kpsFa	PA45B_3244	−	NA	3329327–3330310	Capsule polysaccharide export protein	7.08
neuBa	PA45B_3234	−	NA	3342182–3343222	N,N′-diacetyllegion-aminic acid synthase	7.00
neuDa	PA45B_3233	−	NA	3343219–3343842	Galactoside O-acetyl-transferase monomer	7.00
typAa	PA45B_2253	+	+	4361535–4363358	GTP-binding protein	6.46
kpsUa	PA45B_3241	−	NA	3333240–3333980	Capsule polysaccharide export protein	5.38
rfaHa	PA45B_2283	+	+	4327933–4328421	DNA-binding transcriptional anti-terminator	5.08

^aGene known to be involved in capsule expression.

^bNA, not applicable, because the gene was not mutated.

^cThe log₂ fold change in read counts from the test pool versus the control pool.