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. 2017 Oct 24;7:13922. doi: 10.1038/s41598-017-14382-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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H₂O₂ induces slow pH oscillations. (A) Time traces of 3 individual cells expressing SEP-mRuby after the addition of glucose and hydrogen peroxide. (B) Time traces of 2 individual cells during the slow oscillations while imaging SEP-mRuby and NADH autofluorescence simultaneously. (C) Distribution of frequencies after H₂O₂ addition. The distribution is peaked at 2.4 mHz (416 second period).