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. 2017 Oct 24;7:13896. doi: 10.1038/s41598-017-13713-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gene expression changes in white adipose tissue after ghrelin administration. Normalized gene expression from intra-abdominal white adipose tissue of 14-week old saline treated R6/2 mice (R6/2 NaCl; grey bars), ghrelin treated R6/2 mice (R6/2 Ghrelin; black bars) and saline treated wild type littermates (WT NaCl; white bars). Here, we found ghrelin treatment to have a tendency to normalize leptin mRNA levels in R6/2 mice. We also observed trends towards recovery of reduced R6/2 gene expression for Pparγ, Fabp4, Lpl and Hp, following ghrelin administration. Gene expression was analysed using the ΔΔCt method and normalized to Gusb, Hsp90ab1 and Tbp. N = 8–11/group (n = 5 for the Pparγ R6/2 Ghrelin group). Data represent means ± SEM, *p < 0.05.