Figure 7.

Ghrelin rescues lack of nest building behaviour in R6/2 mice. The nest building deficits present in R6/2 mice in comparison to wild-type mice was rescued by ghrelin administration. Ghrelin was here administrated once daily for 6 weeks starting at 10 weeks. Nesting material was introduced into mice home-cage (mice assessed in pairs), and morning after at 12 and 16 weeks, nest quality was scored as 1–5 as described in Deacon et al.³⁹. Score 1 represents an almost untouched nestlet, for score 3 >50% was shredded but not into a specific nest site, and score 5 represents an almost perfect nest were >90% of the nestlet was used to build the nest as a crater and the walls were higher than the mouse body height. Data represent mean ± SEM, n = 6–8/group. Statistical significance was determined by 2-way ANOVA with Bonferroni post hoc test for multiple comparisons. *p < 0.05, **p < 0.01, ****p < 0.0001.