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. 2017 Oct 24;7:13915. doi: 10.1038/s41598-017-14246-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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GFAP expression of canine (a,c,e) and murine (b,d,f) satellite glial cells (SGCs) supplemented with fibroblast growth factor 2 (FGF), epidermal growth factor, (EGF), ciliary neurotrophic factor (CNTF), heregulin 1β (HRG), and forskolin (Fors). (a,b) Graphs show the percentage of glial fibrillary acidic protein (GFAP)⁺ SGCs with and without supplementation. (c–f) Immunofluorescence double-labelling of SGCs for 2′,3′-cyclic-nucleotide 3′-phosphodiesterase (CNPase; red) and GFAP (green) with bisbenzimide as nuclear counterstain. (c,d) control medium. (e,f) FGF supplementation. *P < 0.05. ***P < 0.001. Bars, 60 μm. Shown are means with standard errors of the mean (SEM).