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. 2017 Oct 20;10:340. doi: 10.3389/fnmol.2017.00340

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Copyright © 2017 Ceolin, Bouquier, Vitre-Boubaker, Rialle, Severac, Valjent, Perroy and Puighermanal.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of Wfs1-CreERT2:RiboTag mouse line. (A) Double immunofluorescence HA (cyan) and NeuN (yellow) in Wfs1-CreERT2:RiboTag mice treated with vehicle (top) or tamoxifen (bottom, 100 mg/kg) for 3 consecutive days and sacrificed 1 week later. (B) Immunoblot of HA of hippocampal homogenates from Wfs1-CreERT2:RiboTag mice treated with vehicle or tamoxifen (100 mg/kg) for 3 consecutive days and sacrificed 1 week later. (C) Specificity of HA-tagged ribosomes immunoprecipitation. Agilent Technologies 2100 Bioanalyzer electropherogram analysis of hippocampal homogenate (input) and immunoprecipitates using anti-HA and control anti-Myc antibodies in Wfs1-CreERT2:RiboTag mice.