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. 2017 Oct 24;7:13859. doi: 10.1038/s41598-017-14313-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SMN protein is released from cells into the extracellular milieu. Various cell lines were plated in 35 mm dishes for 24 h. Media was removed, the cells lysed with protein loading buffer to analyse intracellular protein content (Panel A), and the media subjected to TCA precipitation to analyse extracellular protein content (Panel B). Equal volumes of protein sample were subjected to SDS-PAGE, transferred to a nylon membrane and probed by immunoblot with antibodies to Alix, tubulin, flotillin, or SMN. Data is representative of n = 3.