β-Galactosidase reporter assays on Tac1 TAD GOF mutants. The C. albicans one-hybrid strains expressing individual LexA-Tac1TAD(aa856–981) variants (wild type [yLM579], N972D [yLM581], Δ962–969 [yLM582], N977D [yLM583], G980E [yLM584], or G980W [yLM585]) and one-hybrid strains expressing individual LexA-Tac1ΔDBD(aa130–981) variants (wild-type [yLM568], N972D [yLM573], Δ962–969 [yLM574], N977D [yLM575], G980E [yLM576], or G980W [yLM577]) were tested using β-galactosidase assays. At least three transformants for each construct were measured. Data are reported after normalization to the activation activity of the wild-type variant in each group.