TABLE 1.
Fluconazole MIC in TAC1GOF mutant strains in med3, med15, and cdr1 null backgrounds
| TAC1 allele | Fluconazole MICa (μg/ml) |
||||
|---|---|---|---|---|---|
|
MED3+/+
MED15+/+ |
med3Δ/Δ MED15+/+ |
MED3+/+ med15Δ/Δ CDR1+/+ | |||
| CDR1+/+ | cdr1Δ/Δ | CDR1+/+ | cdr1Δ/Δ | ||
| WT | 0.75 (yLM167) | 0.094 (yLM611) | 0.5–0.75 (yLM232) | —b | 0.5 (yLM240) |
| T225A | 16 (yLM168) | — | 4–6 (yLM233) | — | 6 (yLM241) |
| A736V | 16–24 (yLM169) | 1–1.5 (yLM612) | 6–8 (yLM234) | 1 (yLM615) | 6 (yLM242) |
| G980E | 16–24 (yLM170) | — | 8–12 (yLM235) | — | 8 (yLM243) |
| E461K | 8–12 (yLM490) | — | 4–6 (yLM492) | — | — |
| ΔM677 | 8–12 (yLM502) | — | 4 (yLM504) | — | — |
| N972D | 12 (yLM493) | 0.75–1 (yLM613) | 4 (yLM495) | 1 (yLM616) | — |
| Δ962–969 | 8–12 (yLM499) | — | 3 (yLM501) | — | — |
| N977D | 12–16 (yLM496) | 1 (yLM614) | 4–6 (yLM498) | 1 (yLM617) | — |
a
Fluconazole MICs were measured by Etest at 30°C on YPD plates. Plates were incubated for 36 h before readout. Intermediate values, between scale marks, are presented as intervals. The exact strain used for each MIC measurement is listed in parentheses.
b
—, not determined.