FIG 2.

Time course of induction of whiB7 upon antibiotic exposure. (A) The fold induction of transcript of MAB_3508c in wild-type M. abscessus ATCC 19977 exposed to amikacin (16 μg/ml), clarithromycin (2 μg/ml), erythromycin (10 μg/ml), tetracycline (16 μg/ml), streptomycin (40 μg/ml), and spectinomycin (256 μg/ml) over unexposed samples was determined in previous RNA-Seq studies. The asterisks (*) indicate q values of <0.001 (B) The results were verified by quantitative PCR (qPCR) and expressed as a fold overexpression over unexposed samples. Data represent the mean ± standard deviation (SD), n = 3. sigA was used as an endogenous control.