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Deletion of M. abscessus whiB7. (A) Multiple-sequence alignment of WhiB7 from M. abscessus, M. smegmatis, and M. tuberculosis showing identical residues (*) and conserved motifs. (B) Schematic representation of creating a deletion of MAB_3508c (whiB7) using phage recombineering and unmarking using the Cre-lox system. (C) Three clones were selected (no. 1 to 3), and the whiB7 gene was amplified using the Fcheck and Rcheck primers, followed by confirmation by Sanger sequencing.
