FIG 1.

The cytotoxic effects of hydroxyurea (HU), sampangine, itraconazole, clotrimazole, and terbinafine used alone or in combinations were assessed in S. pombe by standard spot assay. Serial 5-fold dilutions of logarithmically growing wild-type S. pombe were spotted on plates of the rich medium YE6S containing 1.5 mM or 3.0 mM HU in the presence (+) or absence (−) of the heme synthesis inhibitor sampangine or the ergosterol synthesis inhibitors itraconazole, clotrimazole, and terbinafine at the indicated concentrations. The plate on the upper left does not contain any drug. All plates were incubated at 30°C for 3 days and then photographed.