FIG 3.

Combinations of HU with the antifungal azole itraconazole show a highly synergistic cell-killing effect in S. pombe. (A) The cell survival of wild-type S. pombe in the presence of increasing concentrations of HU or itraconazole alone or in various combinations of the two drugs (combos 1 to 4) was assessed by the 96-well plate assay. The cell survival rate and the CI values were calculated as described for Fig. 2. The numbers in bold indicate the drug combinations with very strong synergism. (B) Graphical representation of the cytotoxic effects of HU, itraconazole, and the four combinations of the two drugs shown in panel A. (C) CI-Fa plot.