FIG 4.

Time course analysis of the synergistic cytotoxic effect of HU in combination with SMP, terbinafine, itraconazole, clotrimazole, ketoconazole, or fluconazole in S. pombe. Logarithmically growing wild-type S. pombe was inoculated in YE6S medium on 96-well plates at 3,000 cells/well, and then 10 mM HU and 21 μM SMP (A), 5 mM HU and 0.091 μM terbinafine (B), 10 mM HU and 0.021 μM itraconazole (C), 10 mM HU and 0.043 μM clotrimazole (D), 5 mM HU and 9.4 μM ketoconazole (E), or 10 mM HU and 22.8 μM fluconazole (F) were added to the cell cultures either alone or in combinations. The same amounts of carriers were added for the control. The plates were incubated at 30°C and scanned at the indicated time points to monitor cell survival. All data points are the averages of the readings from three separate wells.