TABLE 4.
Oligonucleotides used in this study
| Primera | Sequenceb |
|---|---|
| atpE_Fw | GTAAGACCGCCGAACTCTTG |
| atpE_Rev | TGGGGATGAGGAAGTTGTTC |
| pepQ_Fw | AAGCCATTGGGTTGTGAGAG |
| pepQ_Rev | GCGGTACAGGTAGGTGGTGT |
| MAB_4312_Fw | GTTATGGGCCGACAGTAGGA |
| MAB_4312_Rev | GATGTGCTTCGGGTTGAAAT |
| MAB_4384_Fw | TTCTGAGTTGGATGTCACGGGCCGGATGA |
| MAB_4384_Rev | CTGCCACGAGATCGACGCCGCTGA |
| MAB_4709c_Fw | CTGGGTCCGAGTAGAAGCTG |
| MAB_4709c_Rev | AGATGCGAAGCGTTCTTGAT |
| atpE_cloning_Fw | TGGCGGACCCCACAATTGTTG |
| atpE_cloning_HindIII_Rev | GAGTGAAAGCTTTTAGCTGGCGCCGGGAGT |
| atpE_D29V_Fw | CCGGTATCGGTGTCGGTATCGCCGGTAA |
| atpE_D29V_Rev | TTACCGGCGATACCGACACCGATACCGG |
| atpE_A64P_Fw | GTCTGGTTGAGGCTCCGTACTTCATCAACCT |
| atpE_A64P_Rev | AGGTTGATGAAGTACGGAGCCTCAACCAGAC |
| pMV5' | CGCCCGGCCAGCGTAAGTAGC |
| pMV3' | GCCTGGCAGTCGATCGTACG |
a
Fw, forward; Rev, reverse.
b
Restriction sites are underlined, and mutated residues are in bold type.