FIG 2.

Opsonophagocytic uptake of bacteria induced by A1124. E. coli ST131 cells were preincubated with 2.5 μg/ml antibody in 5% adsorbed human serum with or without heat treatment. Preopsonized bacteria were incubated with RAW 264.7 murine macrophage cells at an MOI of 1 for 1 h, and uptake was measured by eliminating extracellular bacteria with 40 μg/ml kanamycin for an additional 2 h. Graphs show results of 4 experiments. Statistical analysis was performed using the ratio paired t test.