FIG 4.

Inhibition of hTLR4 signaling by MAb A1124. Effect of A1124 on LPS signaling through hTLR4 was measured with a commercial reporter cell line system (HEK-Blue). Antibody-dependent inhibition of TLR4 signaling induced by purified LPS from ST131 strain 81009. (A) No serum added. (B) In the presence of 4% human serum pool. In all experiments, polymyxin B (PMB) and an isotype-matched MAb with irrelevant specificity served as positive and negative controls, respectively. Graphs show combined results of 2 (A) and 3 (B) experiments, indicating mean ± range.