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Inhibition of the LPS-induced inflammatory cytokine release of cultured macrophages by A1124. RAW 264.7 cells were incubated in the presence of MAb and subsequently stimulated with 10 ng/ml of LPS extracted from E. coli ST131 strain 81009. Supernatants were collected 24 h after the addition of LPS, and the amount of cytokines (TNF-α [A] and IL-6 [B]) was determined by ELISA. Mean ± range of the percent inhibition from 2 independent experiments is shown.
