TABLE 2.
Amino acid variations in the sequences of PDC enzymes from the XDR P. aeruginosa isolates studied
| Wild type or ST | No. of isolates | PDC | Residue at amino acid positiona: |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 7 | 21 | 27 (1) | 55 (29) | 79 (53) | 97 (71) | 105 (79) | 155 (129) | 176 (150) | 205 (179) | 356 (330) | 391 (365) | |||
| PAO1 | PDC-1b | P | T | G | A | R | A | T | Q | L | V | V | G | |
| ST175 | 18 | PDC-1 | ||||||||||||
| ST244 | 6 | PDC-1 | ||||||||||||
| ST235 | 5 | PDC-35 | D | V | A | L | A | |||||||
| ST111 | 2 | PDC-3 | A | |||||||||||
| ST253 | 2 | PDC-34 | D | T | A | R | L | A | ||||||
| ST313 | 2 | PDC-37 | S | D | A | L | I | A | ||||||
| ST455 | 1 | PDC-3 | A | |||||||||||
| ST179 | 1 | PDC-8 | A | R | ||||||||||
| ST274 | 1 | PDC-10 | A | A | ||||||||||
| ST395 | 1 | PDC-8 | A | R | ||||||||||
| ST2221 | 1 | PDC-41c | L | T | K | A | P | Q | L | I | A | |||
| ST2533 | 1 | PDC-5 | Q | A | ||||||||||
| ST2534 | 1 | PDC-31 | A | L | ||||||||||
| ST2535 | 1 | PDC-3 | A | |||||||||||
| ST2536 | 1 | PDC-12 | A | A | L | |||||||||
Number of amino acid residues changed in each PCD. Number in parentheses refers to the mature protein of PAO1, after cleavage of the 26 N-terminal amino acid residues of the signal peptide.
Amino acid residues of P. aeruginosa reference strain PAO1 (http://v2.pseudomonas.com).
This strain has the following additional mutations: D3H, T4A, R5T, F6I, C8N, I12L, L18F, P23S, I25A, D32E (6), S59T (33), L71V (45), G77A (51), Q117R (91), A136G (110), S150A (124), Q174H (148), L200S (174), Q203R (177), Q213H (187), H215F (189), D217R (191), E220A (194), A222Q (196), L223Q (197), A224G (198), Q225L (199), R235H (209), G248A (222), V251L (225), T253S (227), D263E (237), D272E (246), R273K (247), S306D (280), T316A (290), P322A (296), R324K (298), I325V (299), E335D (309), V353L (327), L361V (335), and K396V (370).